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KMID : 0854720070270020098
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Korean Journal of Asthma, Allergy and Clinical Immunology
2007 Volume.27 No. 2 p.98 ~ p.104
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Gene-Gene Interaction between TNF-α -308G/A and IL-13 +2044G/A Polymorphisms with Bronchial Hyperresponsiveness in Korean Children
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Kang Mi-Jin
Kim Hyo-Bin
Jin Hyun-Seung
Lee So-Yeon
Kim Ja-Hyeong
Jang Seong-Ok
Hong Soo-Jong
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Abstract
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Background: Asthma is the most common chronic disease in children. Tumor necrosis factor (TNF)-α is a pivotal pro-Inflammatory cytokine increased in the asthmatic airways. Also, interleukin (IL)-13 is a pivotal immunoregulatory cytokine in asthma pathogenesis. Objective: We assumed that there might be a synergistic effect between TNF-α and IL-13. We analyzed the genotypes of TNF-α and IL-13 polymorphisms in asthmatic patients and control groups, and investigated the association of asthma-related phenotypes with them.
Method: We enrolled 719 asthmatic and 172 control children. Evaluated asthma phenotypes included pulmonary function test, and bronchial hyperresponsiveness (BHR) to methacholine and exercise. We analyzed the genotypes of TNF-α -308G/A and IL-13 +2044G/A polymorphism by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
Result: TNF-α polymorphism was associated with BHR to methacholine (P=0.026). Gene-gene interactions between TNF-α and IL-13 polymorphisms with heterozygote and homozygote of variant genotypes were associated with BHR to methacholine in asthma (P=0.008) and exercise-induced asthma (P=0.005) but were not associated with maximum % fall in FEV1 compared to those with homozygotes of common genotypes.
Conclusion: TNF-α polymorphism was associated with BHR to methacholine. Gene-gene interactions between TNF-α and IL-13 polymorphisms were associated with BHR to methacholine. Therefore, TNF-α and IL-13 polymorphisms may show a synergistic effect in terms of BHR. (Korean J Asthma Allergy Clin Immunol 2007;27:98-104)
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KEYWORD
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Bronchial hyperresponsiveness, Exercise-induced asthma, TNF-α, IL-13, Gene-gene interaction
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